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Now you don't have to just take our word for it.

The proof

There are countless summaries and even full studies available for free on the internet regarding the efficacy of Theobromine and Nano-hydroxyapatite, as well as for most of our ingredients!  I have compiled some here to help in the quest for better health. 

White Structure



(Journal of Clinical Dentistry 2011;22:000–000)


  Remineralization of Early Caries by a Nano-Hydroxyapatite Dentifrice K. Najibfard, DDS, MS, K. Ramalingam, MSc, MPhil, PhD I. Chedjieu, BDS, MPH, B.T. Amaechi, BDS, MS, PhD Department of Comprehensive Dentistry University of Texas Health Science Center at San Antonio



• Objective: The purpose of this randomized, double-blind, crossover, in situ study was to evaluate the efficacy of Nano-hydroxyapatite (nHAP) dentifrices on caries remineralization and demineralization inhibition. • Methods: Three demineralized enamel blocks (A,B,C) and one healthy block (D), cut from each of 30 molars, were exposed respectively to dentifrices of A) 5% nHAP, B) 10% nHAP, C) 1100 ppm fluoride, and D) 10% nHAP via an intra-oral appliance worn by 30 adults in this four-phase study lasting 28 days, per phase. Baseline and post-test mineral loss (Z) and lesion depth (LD) were quantified using microradiography.


• Results: Pair-wise comparison (Baseline versus Test) demonstrated significant (p < 0.001) reductions in Z and LD in A, B, and C. ANOVA showed no significant differences among the three products in percent mineral gain. No demineralization occurred in the sound enamel specimens exposed intra-orally while using 10% nHAP. 


Dentifrices containing nHAP have the potential for the same remineralizing capacity as a fluoride dentifrice and may be employed as an effective alternative to fluoride-containing dentifrices. Based on the findings here, nHAP-containing dentifrices may be recommended for children or those who are concerned about dental fluorosis. Since the remineralizing efficacy of topical fluorides is strictly dependent on the availability of calcium and phosphate ions, nHAP dentifrices are strongly recommended to xerostomic patients with diminished amounts of saliva. Nanohydroxyapatite may have the potential to occlude dentin tubules and, as such, may be useful for the treatment of dentin hypersensitivity, and should be investigated in the future


Grewal N, Sharma N, Kaur N. Surface remineralization potential of nano-hydroxyapatite, sodium mono-fluorophosphate, and amine fluoride containing dentifrices on primary and permanent enamel surfaces: An in vitro study. J Indian Soc Pedod Prev Dent 2018;36:158-66


Background: Organic amine fluorides and nano-hydroxyapatite dentifrices have shown remineralization potential in various studies. However, there is a lack of direct comparison between amine fluoride and nano-hydroxyapatite with conventional inorganic fluorides as sodium mono-fluorophosphate. Aim: The aim of the study is to evaluate remineralizing efficacy of the three dentifrices on both primary and permanent enamel surfaces. Methods: Enamel sections were obtained from 40 sound molars – 20 primary and 20 permanent molars. Five enamel slabs were prepared from these extracted molars stored in artificial saliva, wherein one slab acted as control, second as demineralized (negative control), and other three slabs were brushed with sodium mono-fluorophosphate, amine fluoride, and nano-hydroxyapatite dentifrices, respectively, twice daily for 21 days. Scanning electron microscopy- Energy dispersive spectroscopy (SEM-EDS) analysis for surface morphology and calcium and phosphorus content and Vickers surface microhardness (SMH) values were evaluated at baseline, after demineralization, and post application of the experimental agents. Results: Highly significant changes in calcium phosphorus ratio and SMH values were seen in enamel slabs treated with nano-hydroxyapatite and amine fluoride dentifrice as compared to sodium mono-fluorophosphate in both primary and permanent teeth. Surface morphology of enamel slabs treated with amine fluoride most closely resembled natural enamel morphology, while sodium mono-fluorophosphate-treated surfaces showed globular pattern of remineralization. Deposition of a separate highly mineralized layer over existing surface was seen in nano-hydroxyapatite-treated surfaces. Conclusions: Nano-hydroxyapatite exhibited highest remineralization potential in terms of mineral gain followed by amine fluoride and sodium mono-fluorophosphate dentifrice.


It is time to shift our focus from the traditional dentifrice formulations and adopt more biocompatible and bioactive dentifrices with superior remineralizing efficacy over fluoride-containing dentifrices. Considering the cost-effectiveness of both nano-hydroxyapatite and amine fluoride dentifrice, amine fluoride would provide a cost-effective alternative. However, on the basis of results of the current study, nano-hydroxyapatite dentifrices have shown superior remineralizing efficacy over amine fluoride and sodium monofluorophosphate dentifrices.

This is not the full article but a clip copied from the cover page.  You can access the full article at the link provided below


This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License.


Acta Stomatol Croat. 2021 Mar; 55(1): 18–27.

doi: 10.15644/asc55/1/3

PMCID: PMC8033621

PMID: 33867534


Caries Preventive Effects of Theobromine Containing Toothpaste on Early Childhood Caries: Preliminary Results

Muesser Ahu Durhan,1 Seda Ozsalih,1 Berna Gokkaya,2 Pinar Yildiz Kulan,1 and Betul Kargul1




  "Enamel remineralizing effects of theobromine have received much attention from the clinicians. The aim of this study was to investigate the caries preventive effect of theobromine containing toothpaste on children with early childhood caries (ECC)."


Material and Methods


    "Salivary pH, buffering capacity and frequency of Streptococcus mutans (SM) levels were measured. Each child was assigned either fluoridated or theobromine containing toothpaste. The changes were analyzed using Laser Fluorescence system. Statistical analyses were performed."




  " We included 13 children (mean age 4.25) with 145 teeth in the fluoridated toothpaste (Colgate Kids toothpaste) group; 13 children (mean age 4.46) with 115 teeth in theobromine containing toothpaste (TheodentTMKids toothpaste) group. Both toothpastes demonstrated enamel remineralization and were effective in increasing the buffering capacity and pH (p < 0.05). A statistically significant decrease in S. mutans levels was found in both toothpaste groups (p<0.05)."



   "Both toothpaste group showed a statistically significant amount of enamel remineralization. Since theobromine had the added benefits of increasing the salivary pH and decreasing the S.mutans levels, theobromine containing toothpastes can be considered effective agents in remineralizing white spot lesions and can be used in prevention of early enamel lesions."

Additional quotes from this article:

"It is also a well-known fact that fluoride has a beneficial effect on slowing down the caries progression (4). While the use of fluoride is beneficial for strengthening the enamel, overexposure to fluoride carries the risk of fluorosis. An increased risk of dental and skeletal fluorosis is due to the ingestion of fluoride from different sources and from swallowing toothpaste with fluoride (5). Additionally, it has been disputed whether fluorides interact with ameloblasts, thus having adverse effects on the enamel formation (6)."

"Several studies comparing theobromine and hydroxyapatite were also conducted and the following results were obtained: a study which aimed to analyze and compare the effects of toothpastes containing theobromine and hydroxyapatite on enamel microhardness has found that enamel microhardness was increased significantly after brushing with both Theodent (theobromine) and Pepsodent (HAP) toothpastes. However, the enhancement of enamel hardness in specimens treated with HAP-containing toothpaste was superior to those treated with theobromine toothpaste." 32


This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License.



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